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Anti-CD13 Antibody [ARC0670] (A80859)

Western Blot - Anti-CD13 Antibody [ARC0670] (A80859) - Antibodies.com Immunohistochemistry - Anti-CD13 Antibody [ARC0670] (A80859) - Antibodies.com Immunofluorescence - Anti-CD13 Antibody [ARC0670] (A80859) - Antibodies.com Immunofluorescence - Anti-CD13 Antibody [ARC0670] (A80859) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC0670] antibody to CD13 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-CD13 Antibody [ARC0670]
Description
Rabbit monoclonal [ARC0670] antibody to CD13.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human CD13/ANPEP (P15144).
Sequence
NAGAMENWGLVTYRENSLLFDPLSSSSSNKERVVTVIAHELAHQWFGNLVTIEWWNDLWLNEGFASYVEYLGADYAEPTWNLKDLMVLNDVYRVMAVDALA
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0670
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
160 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, AMPN_HUMAN, ANPEP, AP M, AP N, AP-M, AP-N, APN, CD 13, CD13 antigen, gp150, hAPN, LAP 1, LAP1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, p150, PEPN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD13 Antibody [ARC0670] (A80859) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD13 Antibody [ARC0670] (A80859)
Western blot analysis of extracts of various cell lines, using Anti-CD13 Antibody [ARC0670] (A80859) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-CD13 Antibody [ARC0670] (A80859) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-CD13 Antibody [ARC0670] (A80859)
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-CD13 Antibody [ARC0670] (A80859) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-CD13 Antibody [ARC0670] (A80859) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-CD13 Antibody [ARC0670] (A80859)
Immunofluorescence analysis of THP-1 cells using Anti-CD13 Antibody [ARC0670] (A80859) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-CD13 Antibody [ARC0670] (A80859) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-CD13 Antibody [ARC0670] (A80859)
Immunofluorescence analysis of mouse kidney using Anti-CD13 Antibody [ARC0670] (A80859) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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