Anti-CD14 Antibody [UCHM1] (FITC) (A281873)

Anti-CD14 Antibody [UCHM1] (FITC)

Mouse monoclonal [UCHM1] antibody to CD14 (FITC).

This antibody recognises a cell surface antigen of ~55 kDa, known as CD14.The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative. UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Human, Cynomolgus monkey, Rhesus Monkey, Fish, Trout

Human Thymocytes followed by peripheral blood mononuclear cells.

Mouse

Monoclonal

UCHM1

IgG2a

Protein A affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Human CD14 antibody, clone UCHM1 recognizes a cell surface antigen of ~55 kDa, known as CD14.The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative (Hogg et al. 1984). UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.

CD 14, CD14 antigen, CD14 molecule, CD14_HUMAN, CD_antigen=CD14, LPS-R, Mo2, Monocyte differentiation antigen CD14, Monocyte differentiation antigen CD14 urinary form, Monocyte differentiation antigen CD14, membrane-bound form, Myeloid cell specific leucine rich glycoprotein, Myeloid cell-specific leucine-rich glycoprotein

This product is for research use only. It is not intended for diagnostic or therapeutic use.