Anti-PDGFR alpha Antibody [16A1] (A279042)

Anti-PDGFR alpha Antibody [16A1]

Mouse monoclonal [16A1] antibody to PDGFR alpha.

This antibody recognises CD140a also known as platelet-derived growth factor receptor alpha (PDGFR-a), is a 180 kDa single pass type I transmembrane glycoprotein and a member of the receptor tyrosine kinases (Westermark 1995). CD140a is largely expressed by mesenchymal cells and plays a key role in embryonic organogenesis and development via regulation of differentiation, migration pathways. Upregulation of this receptor has also been associated with various diseases including atherosclerosis, rheumatoid arthritis and nephritis. Upon binding of one of its ligands, PDGF-AA, PDGF-BB or PDGF-AB, the receptor forms either a homo- or hetero-dimer with CD140b. This induces autophosphorylation of the receptors intracellular tyrosine residues which activates downstream signaling pathways such as Ras-MAPK, PI3K and PLC-?. This clone 16A1 has been used in immunocytochemistry experiments to examine the co-localization of CD140a with tryptophan hydroxylase-2 in the myenteric plexus.

Flow Cytometry, ICC

Flow Cytometry: Neat - 1:50, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Human

CD140a-transfected NIH 3T3 cells.

Mouse

Monoclonal

16A1

IgG1

Protein A affinity chromatography of tissue culture supernatant.

>95% (by SDS-PAGE).

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Store undiluted at 4°C. Do not freeze!

Mouse anti Human CD140a, clone 16A1 recognizes CD140a also known as platelet-derived growth factor receptor alpha (PDGFR-a), is a 180 kDa single pass type I transmembrane glycoprotein and a member of the receptor tyrosine kinases (Westermark 1995). CD140a is largely expressed by mesenchymal cells and plays a key role in embryonic organogenesis and development via regulation of differentiation, migration pathways. (Hoch & Sorian 2003). Upregulation of this receptor has also been associated with various diseases including atherosclerosis, rheumatoid arthritis and nephritis (Heldin & Westermark 1999). Upon binding of one of its ligands, PDGF-AA, PDGF-BB or PDGF-AB, the receptor forms either a homo- or hetero-dimer with CD140b. This induces autophosphorylation of the receptors intracellular tyrosine residues which activates downstream signaling pathways such as Ras-MAPK, PI3K and PLC-? (Andrae et al. 2008). This clone 16A1 has been used in immunocytochemistry experiments to examine the co-localization of CD140a with tryptophan hydroxylase-2 in the myenteric plexus (Coyle et al. 2016).

Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a, CD140a antigen, PDGF-R-alpha, PDGFR-2, PDGFR-alpha, PDGFR2, PDGFRA, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, Platelet-derived growth factor receptor alpha, RHEPDGFRA