Anti-CD147 Antibody [ARC2742] (A306188)

Name: Anti-CD147 Antibody [ARC2742]
Brand: Antibodies.com
SKU: A306188

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This product is discontinued

Anti-CD147 Antibody [ARC2742] (A306188) has been discontinued and is no longer available.

View all Anti-CD147 Antibodies.

Specifications

Name

Anti-CD147 Antibody [ARC2742]

Description

Rabbit monoclonal [ARC2742] antibody to CD147.

Applications

WB, IHC

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 286-385 of human CD147/BSG (P35613).

Sequence

HIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLAALWPFLGIVAEVLVLVTIIFIYEKRRKPEDVLDDDDAGSAPLKSSGQHQNDKGKNVRQRNSS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2742

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

5A11 antigen, 5F7, Basigin, Basigin (Ok blood group), BASI_HUMAN, Blood brain barrier HT7 antigen, Bsg, CD 147, CD147 antigen, Collagenase stimulatory factor, EMMPRIN, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, M 6, M6, M6 leukocyte activation antigen, Neurothelin, OK, OK blood group, OK blood group antigen, TCSF, Tumor cell derived collagenase stimulatory factor, Tumor cell-derived collagenase stimulatory factor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-CD147 Antibody [ARC2742] (A306188)

Western blot analysis of extracts of various cell lines, using Anti-CD147 Antibody [ARC2742] (A306188) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-CD147 Antibody [ARC2742] (A306188)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CD147 Antibody [ARC2742] (A306188) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-CD147 Antibody [ARC2742] (A306188)

Immunohistochemistry analysis of paraffin-embedded human colon tissue (negative control sample) using Anti-CD147 Antibody [ARC2742] (A306188) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-CD147 Antibody [ARC2742] (A306188)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-CD147 Antibody [ARC2742] (A306188) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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