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Anti-CD16 + CD32 Antibody [FCR4G8] (PE) (A280459)

This product is discontinued

Anti-CD16 + CD32 Antibody [FCR4G8] (PE) (A280459) has been discontinued and is no longer available.

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Name
Anti-CD16 + CD32 Antibody [FCR4G8] (PE)
Description
Rat monoclonal [FCR4G8] antibody to CD16 + CD32 (PE).
Specificity
This antibody recognises an epitope expressed by the murine low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32). In the mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Mouse
Immunogen
PU5 1.8 IOE7 Balb/c mouse cell line.
Host
Rat
Clonality
Monoclonal
Clone ID
FCR4G8
Isotype
IgG2b
Conjugate

PE

Excitation: 565nm, Emission: 578nm

Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat anti Mouse CD16/CD32 antibody, clone FCR4G8 recognizes an epitope expressed by the murine low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32). In the mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.