Anti-Sialoadhesin / CD169 Antibody [ED3] (A280330)

Anti-Sialoadhesin / CD169 Antibody [ED3]

Mouse monoclonal [ED3] antibody to Sialoadhesin / CD169.

This antibody recognises the rat CD169 cell surface antigen, a ~185 kDa molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.

IHC-Fr, IF, Flow Cytometry, IP

Flow Cytometry: 1:500, IHC-Fr: 1:50 - 1:250, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Rat

Rat spleen cell homogenate.

Mouse

Monoclonal

ED3

IgG2a

Protein A affinity chromatography of tissue culture supernatant.

500 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti rat CD169 antibody, clone ED3 recognises the rat CD169 cell surface antigen, a ~185 kDa molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.