Anti-CD18 Antibody [CA1.4E9] (A279968)

Anti-CD18 Antibody [CA1.4E9]

Mouse monoclonal [CA1.4E9] antibody to CD18.

This antibody recognises the canine CD18 cell surface antigen, also known as the b2 integrin.The tissue and cellular distribution of CD18 in canine tissue closely follows that observed for humans CD18 is expressed by virtually all leucocytes, but more strongly upon monocytes and granulocytes than on lymphocytes. The cross reactivity patterns of recognises an epitope common to a number of mammalian species.Immunoprecipitation experiments using detergent lysates of iodinated peripheral blood leukocytes indicate that clone Ca1.4E9 immunoprecipitates the common 95 kDa beta 2 integrin chain (CD18) along with the non-covalently associated alpha chains at 180 kDa (CD11a ), 165 kDa (CD11b) and 150 kDa (CD11c). Of note is the lack of precipitation of the integrin alpha D chain (CD11d), this is likely due to the almost complete absence of CD11d expression on peripheral blood lymphocytes, in contrast to expression in the splenic red pulp. High CD18 expression is a common feature of lymphoma and hyperplasia in dogs

IP, Flow Cytometry, IHC-Fr, IF

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Canine, Horse, Pig, Human, Bovine, Cat, Mustelid, Mink, Hooded Seal

Mouse

Monoclonal

CA1.4E9

IgG1

Unconjugated

Protein G affinity chromatography of ascites.

1 mg/ml

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Dog CD18 antibody, clone CA1.4E9 recognizes the canine CD18 cell surface antigen, also known as the b2 integrin.The tissue and cellular distribution of CD18 in canine tissue closely follows that observed for humans (Moore et al. 1990) CD18 is expressed by virtually all leucocytes, but more strongly upon monocytes and granulocytes than on lymphocytes. The cross reactivity patterns of Mouse anti Canine CD18, clone CA1.4E9 further indicate that the antibody clone recognizes an epitope common to a number of mammalian species.Immunoprecipitation experiments using detergent lysates of iodinated peripheral blood leukocytes indicate that clone Ca1.4E9 immunoprecipitates the common 95 kDa beta 2 integrin chain (CD18) along with the non-covalently associated alpha chains at 180 kDa (CD11a ), 165 kDa (CD11b) and 150 kDa (CD11c) (Moore et al. 1990). Of note is the lack of precipitation of the integrin alpha D chain (CD11d), this is likely due to the almost complete absence of CD11d expression on peripheral blood lymphocytes, in contrast to expression in the splenic red pulp (Fry et al. 2003). High CD18 expression is a common feature of lymphoma and hyperplasia in dogs (Caniatti et al. 1996)

95 subunit beta, Beta 2 (antigen CD18 (p95), CD 18, Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor), Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement component 3 receptor 3 and 4 subunit, Complement receptor C3 beta subunit, Complement receptor C3 subunit beta, Integrin beta 2, Integrin beta chain beta 2, Integrin beta-2, Integrin beta-2 (ITGB2), Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit), ITB2_HUMAN, ITGB2, LAD, LCAMB, Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C, Leukocyte cell adhesion molecule CD18, LFA 1, LFA1, Lymphocyte function associated antigen 1, Lymphocyte function associated antigen 1 (LFA1), MAC 1, MAC1, Macrophage antigen 1 (mac1) beta, MF17, MFI7, OTTHUMP00000115278, OTTHUMP00000115279, OTTHUMP00000115280, OTTHUMP00000115281, OTTHUMP00000115282