Mouse monoclonal [MEM-48] antibody to CD18 (Alexa Fluor® 700).
Specificity
This antibody recognizes an extracellular epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen. CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Applications
Flow Cytometry, WB
Dilutions
Flow Cytometry: This reagent is designed for analysis of human blood cells using 4µl reagent / 100µl of whole blood or 106 cells in a suspension.
Reactivity
Human
Cross Reactivity
This antibody does not react with Canine.
Immunogen
Leukocytes of a patient suffering from LGL Type Leukaemia.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-48
Isotype
IgG1
Conjugate
Alexa Fluor® 700
Excitation: 702nm, Emission: 723nm
Purification
This antibody is conjugated with Alexa Fluor® 700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 15mM Sodium Azide.
Separation of human lymphocytes (red-filled) from human CD18 negative blood debris (black-dashed) in flow cytometry analysis of human peripheral whole blood stained with Anti-CD18 Antibody [MEM-48] (Alexa Fluor® 700) (4µl reagent / 100µl of peripheral whole blood).
Proteins predicted to interact with CD18
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.