This antibody recognises the rat beta 2 integrin, which is designated CD18. The beta 2 integrin may be associated with the integrin alphaL, alphaX or alphaM subunits. CD18 is expressed by all leucocytes. Mouse anti Rat CD18 antibody has been shown to inhibit homotypic aggregation of PHA blasts. It also blocks binding of rat lymphocytes to purified rat ICAM-1.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Reactivity

Rat

Immunogen

FTL-43.

Host

Mouse

Clonality

Monoclonal

Clone ID

WT.3

Isotype

IgG1

Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification

Protein A affinity chromatography of tissue culture supernatant.

Concentration

100 µg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

General Notes

Mouse anti Rat CD18 antibody, clone WT.3 reacts with the rat beta 2 integrin, which is designated CD18. The beta 2 integrin may be associated with the integrin alphaL, alphaX or alphaM subunits. CD18 is expressed by all leucocytes. Mouse anti Rat CD18 antibody has been shown to inhibit homotypic aggregation of PHA blasts. It also blocks binding of rat lymphocytes to purified rat ICAM-1.

Synonyms

95 subunit beta, Beta 2 (antigen CD18 (p95), CD 18, Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor), Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement component 3 receptor 3 and 4 subunit, Complement receptor C3 beta subunit, Complement receptor C3 subunit beta, Integrin beta 2, Integrin beta chain beta 2, Integrin beta-2, Integrin beta-2 (ITGB2), Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit), ITB2_HUMAN, ITGB2, LAD, LCAMB, Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C, Leukocyte cell adhesion molecule CD18, LFA 1, LFA1, Lymphocyte function associated antigen 1, Lymphocyte function associated antigen 1 (LFA1), MAC 1, MAC1, Macrophage antigen 1 (mac1) beta, MF17, MFI7, OTTHUMP00000115278, OTTHUMP00000115279, OTTHUMP00000115280, OTTHUMP00000115281, OTTHUMP00000115282

Isotype Controls