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Anti-CD21 Antibody (A16181)

Western Blot - Anti-CD21 Antibody (A16181) - Antibodies.com Immunohistochemistry - Anti-CD21 Antibody (A16181) - Antibodies.com Immunofluorescence - Anti-CD21 Antibody (A16181) - Antibodies.com Immunofluorescence - Anti-CD21 Antibody (A16181) - Antibodies.com Immunofluorescence - Anti-CD21 Antibody (A16181) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to CD21 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-CD21 Antibody
Description
Rabbit polyclonal antibody to CD21.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 300-590 of human CR2/CD21 (NP_001006659.1).
Sequence
IVTYTCDPDPEEGVNFILIGESTLRCTVDSQKTGTWSGPAPRCELSTSAVQCPHPQILRGRMVSGQKDRYTYNDTVIFACMFGFTLKGSKQIRCNAQGTWEPSAPVCEKECQAPPNILNGQKEDRHMVRFDPGTSIKYSCNPGYVLVGEESIQCTSEGVWTPPVPQCKVAACEATGRQLLTKPQHQFVRPDVNSSCGEGYKLSGSVYQECQGTIPWFMEIRLCKEITCPPPPVIYNGAHTGSSLEDFPYGTTVTYTCNPGPERGVEFSLIGESTIRCTSNDQERGTWSGPA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
150 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
C3DR, CD 21, Complement C3d receptor, Complement C3d receptor 2, Complement component (3d/Epstein Barr virus) receptor 2, Complement receptor type 2, CR, Cr2, CR2_HUMAN, CVID7, EBV receptor, EBV-R, Epstein Barr virus receptor, Epstein-Barr virus receptor, EVBR, SLEB9
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD21 Antibody (A16181) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD21 Antibody (A16181)
Western blot analysis of extracts of various cell lines, using Anti-CD21 Antibody (A16181) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry - Anti-CD21 Antibody (A16181) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-CD21 Antibody (A16181)
Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-CD21 Antibody (A16181) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-CD21 Antibody (A16181) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-CD21 Antibody (A16181)
Immunofluorescence analysis of rat spleen using Anti-CD21 Antibody (A16181) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-CD21 Antibody (A16181) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-CD21 Antibody (A16181)
Immunofluorescence analysis of human spleen using Anti-CD21 Antibody (A16181) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-CD21 Antibody (A16181)? Please let us know so that we can list the citation on this page.

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