Mouse monoclonal [S-HCL-1] antibody to CD22 (Alexa Fluor® 700).
Specificity
This antibody recognizes an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension.
Reactivity
Human
Immunogen
Whole hairy cell leukemia cells and membrane preparation.
Host
Mouse
Clonality
Monoclonal
Clone ID
S-HCL-1
Isotype
IgG2b
Light Chains
kappa
Conjugate
Alexa Fluor® 700
Excitation: 702nm, Emission: 723nm
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.