Anti-CD22 Antibody [S-HCL-1] (Pacific Blue™) (A285907)

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This product is discontinued

Anti-CD22 Antibody [S-HCL-1] (Pacific Blue™) (A285907) has been discontinued and is no longer available.

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Specifications

Name

Anti-CD22 Antibody [S-HCL-1] (Pacific Blue™)

Description

Mouse monoclonal [S-HCL-1] antibody to CD22 (Pacific Blue™).

Specificity

This antibody recognizes an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension.

Reactivity

Human

Immunogen

Whole hairy cell leukemia cells and membrane preparation.

Host

Mouse

Clonality

Monoclonal

Clone ID

S-HCL-1

Isotype

IgG2b

Light Chains

kappa

Conjugate

Pacific Blue™

Excitation: 410nm, Emission: 455nm

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Store in the dark at 2-8°C. Do not freeze!

Synonyms

B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, Sialic acid-binding Ig-like lectin 2, Siglec-2, SIGLEC2, T-cell surface antigen Leu-14

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Flow Cytometry - Anti-CD22 Antibody [S-HCL-1] (Pacific Blue™) (A285907)

Flow cytometry surface staining pattern of human peripheral whole blood stained with Anti-CD22 Antibody [S-HCL-1] (Pacific Blue™) (4 µl reagent / 100 µl of peripheral whole blood).

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Flow Cytometry - Anti-CD22 Antibody [S-HCL-1] (Pacific Blue™) (A285907)

Separation of human CD22 positive lymphocytes (red-filled) from CD22 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-CD22 Antibody [S-HCL-1] (Pacific Blue™) (4 µl reagent / 100 µl of peripheral whole blood).