Anti-CD235a Antibody [JC159] (Pacific Blue™) (A285910)

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This product is discontinued

Anti-CD235a Antibody [JC159] (Pacific Blue™) (A285910) has been discontinued and is no longer available.

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Specifications

Name

Anti-CD235a Antibody [JC159] (Pacific Blue™)

Description

Mouse monoclonal [JC159] antibody to CD235a (Pacific Blue™).

Specificity

This antibody recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. This antibody does not react with glycophorin B.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension.

Reactivity

Rat, Human

Immunogen

Membrane preparation from splenic hairy cell leukemia.

Host

Mouse

Clonality

Monoclonal

Clone ID

JC159

Isotype

IgG1

Conjugate

Pacific Blue™

Excitation: 410nm, Emission: 455nm

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Store in the dark at 2-8°C. Do not freeze!

Synonyms

Glycophorin-A, GPA, GYPA, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha

Isotype Controls

Mouse IgG1 [MOPC-21] (Pacific Blue™) (A18842)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Flow Cytometry - Anti-CD235a Antibody [JC159] (Pacific Blue™) (A285910)

Flow cytometry surface staining pattern of human peripheral whole blood stained with Anti-CD235a Antibody [JC159] (Pacific Blue™) (4 µl reagent / 100 µl of peripheral whole blood).

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Flow Cytometry - Anti-CD235a Antibody [JC159] (Pacific Blue™) (A285910)

Separation of human CD235a positive erythrocytes (red-filled) from lymphocytes(black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-CD235a Antibody [JC159] (Pacific Blue™) (4 µl reagent / 100 µl of peripheral whole blood).