Mouse monoclonal [MEM-181] antibody to CD25 (Pacific Blue).
Specificity
This antibody reacts with an extracellular epitope of CD25, a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes / macrophages, and on CD4+ T lymphocytes (T regulatory cells). It is lost on resting B and T lymphocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: This reagent is designed for analysis of human blood cells using 4µl reagent / 100µl of whole blood or 106 cells in a suspension.
Reactivity
Human
Immunogen
PHA-activated peripheral blood leucocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-181
Isotype
IgG1
Conjugate
Pacific Blue
Excitation: 410nm, Emission: 455nm
Purification
This antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 15mM Sodium Azide.