CD253 Cell Based ELISA Kit (A103694)

CD253 Cell Based ELISA Kit

Cell-based (qualitative)

This CD253 Cell Based ELISA Kit allows for the detection of CD253 and the effects that certain stimulation conditions have on CD253 expression in different cell lines. Qualitative determination of CD253 concentration is achieved by an indirect ELISA format. In essence, the CD253 is captured by Anti-CD253 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this CD253 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Colorimetric

> 5000 Cells

4 hours 30 minutes

Human

Store at + 4°C. Stable for 6 months.

Apo 2 ligand, APO 2L, Apo-2 ligand, Apo-2L, APO2L, CD253 antigen, Chemokine tumor necrosis factor ligand superfamily member 10, Protein TRAIL, TL2, TNF Related Apoptosis Inducing Ligand, TNF related apoptosis inducing ligand TRAIL, TNF-related apoptosis-inducing ligand, TNF10_HUMAN, TNFSF10, TRAIL, Tumor necrosis factor (ligand) family member 10, Tumor Necrosis Factor (ligand) Superfamily Member 10, Tumor necrosis factor apoptosis inducing ligand splice variant delta, Tumor necrosis factor ligand superfamily member 10