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Anti-CD27 Antibody [ARC0625] (A81178)

Western Blot - Anti-CD27 Antibody [ARC0625] (A81178) - Antibodies.com Immunofluorescence - Anti-CD27 Antibody [ARC0625] (A81178) - Antibodies.com Immunofluorescence - Anti-CD27 Antibody [ARC0625] (A81178) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC0625] antibody to CD27 for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
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Email
orders@antibodies.com

Name
Anti-CD27 Antibody [ARC0625]
Description
Rabbit monoclonal [ARC0625] antibody to CD27.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD27 (P26842).
Sequence
MARPHPWWLCVLGTLVGLSATPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITA
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0625
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CD 27, CD27 antigen, CD27 molecule, CD27L receptor, CD27_HUMAN, LPFS2, MGC20393, OTTHUMP00000238557, S152, T cell activation antigen CD27, T cell antivation antigen S152, T-cell activation antigen CD27, T14, TNFRSF 7, TNFRSF7, TNFSF7, Tp 55, Tp55, Tumor necrosis factor receptor superfamily member 7
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD27 Antibody [ARC0625] (A81178) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD27 Antibody [ARC0625] (A81178)
Western blot analysis of extracts of various cell lines, using Anti-CD27 Antibody [ARC0625] (A81178) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunofluorescence - Anti-CD27 Antibody [ARC0625] (A81178) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-CD27 Antibody [ARC0625] (A81178)
Immunofluorescence analysis of Jurkat cells using Anti-CD27 Antibody [ARC0625] (A81178) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-CD27 Antibody [ARC0625] (A81178) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-CD27 Antibody [ARC0625] (A81178)
Immunofluorescence analysis of rat thymus using Anti-CD27 Antibody [ARC0625] (A81178) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-CD27 Antibody [ARC0625] (A81178)? Please let us know so that we can list the citation on this page.

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