Anti-CD274 Antibody [29E.2A3] (Alexa Fluor® 700) (A121751)

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This product is discontinued

Anti-CD274 Antibody [29E.2A3] (Alexa Fluor® 700) (A121751) has been discontinued and is no longer available.

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Specifications

Name

Anti-CD274 Antibody [29E.2A3] (Alexa Fluor® 700)

Description

Mouse monoclonal [29E.2A3] antibody to CD274 (Alexa Fluor® 700).

Specificity

This antibody recognizes an extracellular epitope of CD274, a 40 kDa type I transmembrane protein expressed by dendritic cells, activated T cells, activated monocytes, and in various tissues, above all in heart and skeletal muscle, placenta and lung, and in many cancer cells, including: T cell lymphomas, melanomas, and glioblastomas.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: This reagent is designed for analysis of human blood cells using 4µl reagent / 100µl of whole blood or 10⁶ cells in a suspension.

Reactivity

Human, Non-Human Primates

Immunogen

Full length human CD274.

Host

Mouse

Clonality

Monoclonal

Clone ID

29E.2A3

Isotype

IgG2b

Conjugate

Alexa Fluor® 700

Excitation: 702nm, Emission: 723nm

Purification

This antibody is conjugated with Alexa Fluor® 700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 15mM Sodium Azide.

Storage

Store in the dark at 2-8°C. Do not freeze!

Synonyms

B7 homolog 1, B7-H1, B7H1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Flow Cytometry - Anti-CD274 Antibody [29E.2A3] (Alexa Fluor® 700) (A121751)

Flow cytometric analysis of human PHA stimulated peripheral blood mononuclear cells stained with Anti-CD274 Antibody [29E.2A3] (Alexa Fluor® 700) (4µl reagent per milion cells in 100µl of cell suspension).

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Flow Cytometry - Anti-CD274 Antibody [29E.2A3] (Alexa Fluor® 700) (A121751)

Separation of human lymphocytes (red-filled) from cellular debris (black-dashed) in flow cytometry analysis of human PHA stimulated peripheral blood mononuclear cells stained with Anti-CD274 Antibody [29E.2A3] (Alexa Fluor® 700) (4µl reagent per milion cells in 100µl of cell suspension).