Anti-CD3 Antibody [CA17.2A12] (FITC) (A279975)

$590

Mouse monoclonal [CA17.2A12] antibody to CD3 (FITC) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-CD3 Antibody [CA17.2A12] (FITC)
Description
Mouse monoclonal [CA17.2A12] antibody to CD3 (FITC).
Specificity
This antibody recognises the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily. Mouse anti Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood. Note: MCA1774F should not be used with MCA1781PE (mouse anti canine B-cells), in dual colour FC, due to non-specific interactions between the two reagents.
Reactivity
Canine
Immunogen
Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-90.
Host
Mouse
Clonality
Monoclonal
Clone ID
CA17.2A12
Isotype
IgG1
Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification
Protein G affinity chromatography of ascites.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Dog CD3 antibody, clone CA17.2A12 recognizes the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily. Mouse anti Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin (Miniscalco et al. 2003).
Synonyms
4930549J05Rik, A430104F18Rik, AW552088, Cd247, CD247 antigen, CD247 antigen, zeta subunit, CD247 molecule, CD3 antigen, delta subunit, CD3 delta, CD3 epsilon, CD3 eta, CD3 gamma, CD3 molecule delta polypeptide, CD3 molecule, epsilon polypeptide, CD3 molecule, gamma polypeptide, CD3 zeta, CD3-DELTA, CD3d, CD3D antigen delta polypeptide, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule delta, CD3d molecule delta CD3 TCR complex, CD3d molecule, delta (CD3-TCR complex), CD3D_HUMAN, CD3E, CD3e antigen, CD3E antigen epsilon polypeptide, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule epsilon, CD3e molecule epsilon CD3 TCR complex, CD3e molecule, epsilon (CD3-TCR complex), CD3epsilon, CD3G, CD3g antigen, CD3G antigen gamma polypeptide, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule gamma, CD3g molecule gamma CD3 TCR complex, CD3g molecule, gamma (CD3-TCR complex), CD3H, CD3Q, CD3Z, CD3zeta, Ctg3, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD19, Leu-4, MGC138597, OKT3, delta chain, OTTHUMP00000032544, T cell receptor, T cell receptor T3 delta chain, T cell receptor T3 gamma chain, T cell receptor T3 zeta chain, T cell receptor zeta chain, T cell surface glycoprotein CD3, T cell surface glycoprotein CD3 delta chain, T cell surface glycoprotein CD3 epsilon chain, T cell surface glycoprotein CD3 gamma chain, T cell surface glycoprotein CD3 zeta chain, T-cell antigen receptor complex, delta subunit of T3, T-cell antigen receptor complex, epsilon subunit of T3, T-cell antigen receptor complex, gamma subunit of T3, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 delta chain, T-cell receptor T3 gamma chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 gamma chain, T3, T3d, T3e, T3g, T3z, TCRE, TCRk, Tcrz, TCRzeta
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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