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Anti-CD3 Antibody [UCHT1] (FITC) / Anti-HLA Class II Antibody [WR18] (PE) (A281887)

This product is discontinued

Anti-CD3 Antibody [UCHT1] (FITC) / Anti-HLA Class II Antibody [WR18] (PE) (A281887) has been discontinued and is no longer available.

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Name
Anti-CD3 Antibody [UCHT1] (FITC) / Anti-HLA Class II Antibody [WR18] (PE)
Description
Dual color: Mouse monoclonal [UCHT1] antibody to CD3 (FITC) + Mouse monoclonal [WR18] antibody to HLA Class II (PE).
Specificity
This antibody recognises the human CD3 cell surface antigen, expressed by T lymphocytes. Clone WR18 recognises a common determinant on the beta chain of the human HLA Class 2 molecules HLA-DR, DP and DQ. This antigen is expressed on B lymphocytes, monocytes and on activated T lymphocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood
Reactivity
Human
Host
Mouse
Clonality
Oligoclonal
Clone ID
UCHT1 + WR18
Conjugate

FITC + PE

Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 0.5 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Human CD3:FITC/HLA-CLASS 2:RPE Dual color reagent is designed to permit the simultaneous enumeration of CD3 and HLA Class 2 expressing cells. It is particularly useful in the assessment of HLA Class 2 expression on T lymphocytes. Clone UCHT1 recognizes the human CD3 cell surface antigen, expressed by T lymphocytes. Clone WR18 recognises a common determinant on the beta chain of the human HLA Class 2 molecules HLA-DR, DP and DQ. This antigen is expressed on B lymphocytes, monocytes and on activated T lymphocytes.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.