Anti-CD300a Antibody [P192] (A281532)

Anti-CD300a Antibody [P192]

Mouse monoclonal [P192] antibody to CD300a.

This antibody recognises human CD300a, otherwise known as IRp60 or inhibitory receptor protein 60. CD300a is a ~60kDa transmembrane glycoprotein expressed by natural killer cells (NK), T and B cell subsets, monocytes, neutrophils, macrophages, granulocytes, dendritic cells (DC) and mast cells.Cross-linking of CD300a on NK results in a down-regulation of their cytolytic activity, whilst cross-linking of CD300a on mast cells, inhibits IgE-induced degranulation and SCF-mediated survival. Furthermore, cross-linking of CD300a on the surface of eosinophils significantly inhibits their survival, chemotaxis and activation in response to certain inflammatory cytokines, whilst eosinophil-derived MBP (major basic protein) and EDN (neurotoxin), can down-regulate CD300a expression on mast cells in vitro.

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Human

Polyclonal population of NK cells.

Mouse

Monoclonal

P192

IgG2a

Unconjugated

Protein G affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD300a antibody, clone P192 recognizes human CD300a, otherwise known as IRp60 or inhibitory receptor protein 60. CD300a is a ~60kDa transmembrane glycoprotein expressed by natural killer cells (NK), T and B cell subsets, monocytes, neutrophils, macrophages, granulocytes, dendritic cells (DC) and mast cells.Cross-linking of CD300a on NK results in a down-regulation of their cytolytic activity, whilst cross-linking of CD300a on mast cells, inhibits IgE-induced degranulation and SCF-mediated survival. Furthermore, cross-linking of CD300a on the surface of eosinophils significantly inhibits their survival, chemotaxis and activation in response to certain inflammatory cytokines, whilst eosinophil-derived MBP (major basic protein) and EDN (neurotoxin), can down-regulate CD300a expression on mast cells in vitro.

CD300 antigen, CD300 antigen-like family member A, CD300a antigen, CD300a molecule, CLM-8, CLM8_HUMAN, CMRF 35 H9, CMRF-35-H9, CMRF35-H, CMRF35-H9, CMRF35-like molecule 8, CMRF35H, CMRF35H leukocyte immunoglobulin like receptor, CMRF35H9, IgSF12, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, IRC1, IRC1/IRC2, IRC2, IRp60, Leukocyte membrane antigen, Mast cell derived paired immunoglobulin like receptor 1, NK inhibitory receptor, Polymeric immunoglobulin receptor 4