Anti-CD300a Antibody [P192] (FITC) (A281530)

$420

Mouse monoclonal [P192] antibody to CD300a (FITC) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-CD300a Antibody [P192] (FITC)
Description
Mouse monoclonal [P192] antibody to CD300a (FITC).
Specificity
This antibody recognises human CD300a, otherwise known as IRp60 or inhibitory receptor protein 60. CD300a is a ~60kDa transmembrane glycoprotein expressed by natural killer cells (NK), T and B cell subsets, monocytes, neutrophils, macrophages, granulocytes, dendritic cells (DC) and mast cells.Cross-linking of CD300a on NK results in a down-regulation of their cytolytic activity, whilst cross-linking of CD300a on mast cells, inhibits IgE-induced degranulation and SCF-mediated survival. Furthermore, cross-linking of CD300a on the surface of eosinophils significantly inhibits their survival, chemotaxis and activation in response to certain inflammatory cytokines, whilst eosinophil-derived MBP (major basic protein) and EDN (neurotoxin), can down-regulate CD300a expression on mast cells in vitro.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
Reactivity
Human
Immunogen
Polyclonal population of NK cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
P192
Isotype
IgG2a
Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human CD300a antibody, clone P192 recognizes human CD300a, otherwise known as IRp60 or inhibitory receptor protein 60. CD300a is a ~60kDa transmembrane glycoprotein expressed by natural killer cells (NK), T and B cell subsets, monocytes, neutrophils, macrophages, granulocytes, dendritic cells (DC) and mast cells.Cross-linking of CD300a on NK results in a down-regulation of their cytolytic activity, whilst cross-linking of CD300a on mast cells, inhibits IgE-induced degranulation and SCF-mediated survival. Furthermore, cross-linking of CD300a on the surface of eosinophils significantly inhibits their survival, chemotaxis and activation in response to certain inflammatory cytokines, whilst eosinophil-derived MBP (major basic protein) and EDN (neurotoxin), can down-regulate CD300a expression on mast cells in vitro.
Synonyms
CD300 antigen, CD300 antigen-like family member A, CD300a antigen, CD300a molecule, CLM-8, CLM8_HUMAN, CMRF 35 H9, CMRF-35-H9, CMRF35-H, CMRF35-H9, CMRF35-like molecule 8, CMRF35H, CMRF35H leukocyte immunoglobulin like receptor, CMRF35H9, IgSF12, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, IRC1, IRC1/IRC2, IRC2, IRp60, Leukocyte membrane antigen, Mast cell derived paired immunoglobulin like receptor 1, NK inhibitory receptor, Polymeric immunoglobulin receptor 4
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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