Anti-CD31 Antibody [ARC50362] (A305352)

Name: Anti-CD31 Antibody [ARC50362]
Brand: Antibodies.com
SKU: A305352
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC50362] antibody to CD31 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Tags:

Embryonic Stem Cell Marker Endothelial Stem Cell Marker Hematopoietic Stem Cell Marker Intercellular Junction Marker

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Specifications

Name

Anti-CD31 Antibody [ARC50362]

Description

Rabbit monoclonal [ARC50362] antibody to CD31.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:500-1:1,000, ICC/IF: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 630-738 of CD31/PECAM1 (NP_000433.4).

Sequence

AKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVRNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50362

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

135 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Adhesion molecule, CD31 antigen, CD31 EndoCAM, EndoCAM, FLJ34100, FLJ58394, GPIIA, GPIIA', GPIIA'', HEC7, Leukocyte surface protein, PECA1, PECA1_HUMAN, PECAM, PECAM 1, PECAM 1 CD31 EndoCAM, PECAM-1, Pecam1, Platelet and endothelial cell adhesion molecule 1, Platelet endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-CD31 Antibody [ARC50362] (A305352)

Western blot analysis of extracts of various cell lines, using Anti-CD31 Antibody [ARC50362] (A305352) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-CD31 Antibody [ARC50362] (A305352)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CD31 Antibody [ARC50362] (A305352) at a dilution of 1:600(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-CD31 Antibody [ARC50362] (A305352)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-CD31 Antibody [ARC50362] (A305352) at a dilution of 1:600(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-CD31 Antibody [ARC50362] (A305352)

Immunofluorescence analysis of human placentla using Anti-CD31 Antibody [ARC50362] (A305352) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).