This antibody recognizes CD32 on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).

Applications

Flow Cytometry

Dilutions

Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Purified glycosylated recombinant human Fc gamma R IIa2.

Host

Mouse

Clonality

Monoclonal

Clone ID

3D3

Isotype

IgG1

Light Chains

kappa

Conjugate

Excitation: 565nm, Emission: 578nm

Purification

This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Synonyms

CD32A, CD32B, CD32C, CDw32, Fc fragment of IgG low affinity IIa receptor, Fc fragment of IgG low affinity IIa receptor for (CD32), Fc fragment of IgG low affinity IIb receptor, Fc fragment of IgG low affinity IIb receptor for (CD32), Fc fragment of IgG low affinity IIc receptor, Fc fragment of IgG low affinity IIc receptor for (CD32), Fc fragment of IgG, low affinity II, receptor for (CD32), Fc fragment of IgG, low affinity IIa, receptor (CD32), Fc fragment of IgG, low affinity IIb, receptor (CD32), Fc gamma receptor IIB, Fc gamma receptor IIC, Fc gamma RII a, Fc gamma RII b, Fc gamma RII c, Fc gamma RIIb, Fc gamma RIIc, Fc receptor, IgG, low affinity IIb, Fc-gamma RII-a, Fc-gamma RII-b, Fc-gamma RII-c, Fc-gamma-RIIa, FC-gamma-RIIB, Fc-GAMMA-RIIC, FCG2, FCG2A_HUMAN, FcGR, FCGR2, FCGR2A, FCGR2A1, FCGR2B, FCGR2C, Fcgr3, Fcgr3a, FcgRII, Fcr-2, Fcr-3, FCRII, FcRII a, FcRII b, FcRII c, FcRII-a, FcRIIC, Fc[g]RII, IGFR2, IgG Fc receptor II a, IgG Fc receptor II b, IgG Fc receptor II beta, IgG Fc receptor II c, IgG Fc receptor II-a, IgG Fc receptor II-b, IgG Fc receptor II-c, Immunoglobulin G Fc receptor II, Immunoglobulin G Fc receptor II c, Low affinity immunoglobulin gamma Fc region receptor II, Low affinity immunoglobulin gamma Fc region receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-b, Low affinity immunoglobulin gamma Fc region receptor II-c, Ly-17, Ly-m20, LyM-1, Lymphocyte antigen 17

Isotype Controls