Anti-CD32 Antibody [CCG36] (PE) (A280073)

Anti-CD32 Antibody [CCG36] (PE)

Mouse monoclonal [CCG36] antibody to CD32 (PE).

This antibody recognises the bovine homologue of human CD32, one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. Bovine CD32 is a single pass type 1 membrane protein of approximately 32kDa, expressed on the cell surface of most cells including B-lymphocytes, monocytes, neutrophils and afferent veiled lymph dendritic cells Chattha, K. et al. 2010. It has been shown that expression of bovine CD32 is higher on macrophages than on neutrophils.CD32 can function in an inhibitory capacity to antibody production and is the low affinity Fc receptor for IgG (FcRII), binding to the Fc region of immunoglobulin gamma Chattha et al. 2009.recognises ovine CD32 while clone CCG39 recognises only bovine CD32.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Bovine, Sheep

Bovine Fc gamma RII-transfected COS7 cells.

Mouse

Monoclonal

CCG36

IgG1

PE

Excitation: 565nm, Emission: 578nm

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light.

Mouse anti Bovine CD32 antibody, clone CCG36 recognizes the bovine homologue of human CD32, one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. Bovine CD32 is a single pass type 1 membrane protein of approximately 32kDa, expressed on the cell surface of most cells including B-lymphocytes, monocytes, neutrophils and afferent veiled lymph dendritic cells Chattha, K. et al. 2010. It has been shown that expression of bovine CD32 is higher on macrophages than on neutrophils.CD32 can function in an inhibitory capacity to antibody production and is the low affinity Fc receptor for IgG (FcRII), binding to the Fc region of immunoglobulin gamma Chattha et al. 2009.Mouse anti Bovine CD32, clone CCG36 is one of a number of anti bovine CD32 reagents available from Bio-Rad, clone CCG36 is of interest in that it also recognizes ovine CD32 while clone CCG39 recognizes only bovine CD32.

CD32A, CD32B, CD32C, CDw32, Fc fragment of IgG low affinity IIa receptor, Fc fragment of IgG low affinity IIa receptor for (CD32), Fc fragment of IgG low affinity IIb receptor, Fc fragment of IgG low affinity IIb receptor for (CD32), Fc fragment of IgG low affinity IIc receptor, Fc fragment of IgG low affinity IIc receptor for (CD32), Fc fragment of IgG, low affinity II, receptor for (CD32), Fc fragment of IgG, low affinity IIa, receptor (CD32), Fc fragment of IgG, low affinity IIb, receptor (CD32), Fc gamma receptor IIB, Fc gamma receptor IIC, Fc gamma RII a, Fc gamma RII b, Fc gamma RII c, Fc gamma RIIb, Fc gamma RIIc, Fc receptor, IgG, low affinity IIb, Fc-gamma RII-a, Fc-gamma RII-b, Fc-gamma RII-c, Fc-gamma-RIIa, FC-gamma-RIIB, Fc-GAMMA-RIIC, FCG2, FCG2A_HUMAN, FcGR, FCGR2, FCGR2A, FCGR2A1, FCGR2B, FCGR2C, Fcgr3, Fcgr3a, FcgRII, Fcr-2, Fcr-3, FCRII, FcRII a, FcRII b, FcRII c, FcRII-a, FcRIIC, Fc[g]RII, IGFR2, IgG Fc receptor II a, IgG Fc receptor II b, IgG Fc receptor II beta, IgG Fc receptor II c, IgG Fc receptor II-a, IgG Fc receptor II-b, IgG Fc receptor II-c, Immunoglobulin G Fc receptor II, Immunoglobulin G Fc receptor II c, Low affinity immunoglobulin gamma Fc region receptor II, Low affinity immunoglobulin gamma Fc region receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-b, Low affinity immunoglobulin gamma Fc region receptor II-c, Ly-17, Ly-m20, LyM-1, Lymphocyte antigen 17

This product is for research use only. It is not intended for diagnostic or therapeutic use.