Anti-CD33 Antibody (A13841)

Name: Anti-CD33 Antibody
Brand: Antibodies.com
SKU: A13841
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to CD33 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Hematopoietic Stem Cell Marker

Specifications

Name

Anti-CD33 Antibody

Description

Rabbit polyclonal antibody to CD33.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 49-259 of human SIGLEC3/CD33 (NP_001763.3).

Sequence

YYDKNSPVHGYWFREGAIISRDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVH

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CD 33, CD33 antigen, CD33 antigen (gp67), CD33 molecule, CD33_HUMAN, FLJ00391, gp67, My9, Myeloid cell surface antigen CD33, Myeloid cell surface antigen CD33 precursor, Myeloid differentiation antigen CD33, p67, Sialic acid binding Ig like lectin 3, Sialic acid binding immunoglobulin like lectin 3, Sialic acid-binding Ig-like lectin 3, SIGLEC 3, Siglec-3, SIGLEC3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-CD33 Antibody (A13841)

Western blot analysis of extracts of various cell lines, using Anti-CD33 Antibody (A13841) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Immunohistochemistry - Anti-CD33 Antibody (A13841)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-CD33 Antibody (A13841) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-CD33 Antibody (A13841)

Immunofluorescence analysis of K-562 cells using Anti-CD33 Antibody (A13841) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-CD33 Antibody (A13841)

Immunofluorescence analysis of RAW264. 7 cells using Anti-CD33 Antibody (A13841) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).