Anti-CD33 Antibody [WM53] (Pacific Blue™) (A285908)

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This product is discontinued

Anti-CD33 Antibody [WM53] (Pacific Blue™) (A285908) has been discontinued and is no longer available.

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Specifications

Name

Anti-CD33 Antibody [WM53] (Pacific Blue™)

Description

Mouse monoclonal [WM53] antibody to CD33 (Pacific Blue™).

Specificity

This antibody reacts with an extracellular epitope of CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension.

Reactivity

Non-Human Primates, Human

Immunogen

Human AML cells.

Host

Mouse

Clonality

Monoclonal

Clone ID

WM53

Isotype

IgG1

Conjugate

Pacific Blue™

Excitation: 410nm, Emission: 455nm

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Store in the dark at 2-8°C. Do not freeze!

Synonyms

gp67, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, SIGLEC3

Isotype Controls

Mouse IgG1 [MOPC-21] (Pacific Blue™) (A18842)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Flow Cytometry - Anti-CD33 Antibody [WM53] (Pacific Blue™) (A285908)

Flow cytometry surface staining pattern of human peripheral whole blood stained with Anti-CD33 Antibody [WM53] (Pacific Blue™) (4 µl reagent / 100 µl of peripheral whole blood).

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Flow Cytometry - Anti-CD33 Antibody [WM53] (Pacific Blue™) (A285908)

Separation of human CD33 positive monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-CD33 Antibody [WM53] (Pacific Blue™) (4 µl reagent / 100 µl of peripheral whole blood).