Anti-CD35 Antibody [E11] - BSA and Azide free (A280300)

$800

Mouse monoclonal [E11] antibody to CD35 for IHC-Fr, IHC-P and Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-CD35 Antibody [E11] - BSA and Azide free
Description
Mouse monoclonal [E11] antibody to CD35.
Specificity
This antibody recognises complement receptor type 1, also known as CD35 or the C3b/C4b receptor. CD35 a single chain, type I single pass transmembrane glycoprotein which exists in four allotypic forms (A,B,C,D) of ~190 kDa, ~220 kDa, ~160 kDa and ~250 kDa respectively. CD35 is expressed by granulocytes, monocytes, B cells and some T cells. Functionally, CD35 acts as a receptor for the C3b component of complement. Mouse anti Human CD35 antibody, clone E11 is suitable for use in Western Blotting.
Applications
IHC-Fr, IHC-P, Flow Cytometry
Dilutions
Flow Cytometry: 1:50 - 1:200, IHC-Fr: 1 µg/ml - 10 µg/ml, IHC-P: 1 µg/ml - 10 µg/ml, Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood.
Reactivity
Human, Cynomolgus monkey, Rhesus Monkey, Baboon
Immunogen
Human acute monocytic leukaemia cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
E11
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Human CD35 antibody, clone E11 recognizes complement receptor type 1, also known as CD35 or the C3b/C4b receptor. CD35 a single chain, type I single pass transmembrane glycoprotein which exists in four allotypic forms (A,B,C,D) of ~190 kDa, ~220 kDa, ~160 kDa and ~250 kDa respectively (Khera et al. 2009). CD35 is expressed by granulocytes, monocytes, B cells and some T cells. Functionally, CD35 acts as a receptor for the C3b component of complement.Mouse anti Human CD35 antibody, clone E11 is suitable for use in Western Blotting (Birmingham et al. 1996).
Synonyms
C3 binding protein, C3b/C4b receptor, C3BR, C4BR, CD 35, CD35 antigen, complement component (3b/4b) receptor 1 (Knops blood group), complement component (3b/4b) receptor 1 including Knops blood group system, Complement component receptor 1, Complement receptor 1, Complement receptor type 1, CR 1, CR1, CR1_HUMAN, KN, Knops blood group antigen
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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