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Anti-CD4 Antibody (A91607)

Western Blot - Anti-CD4 Antibody (A91607) - Antibodies.com Western Blot - Anti-CD4 Antibody (A91607) - Antibodies.com Western Blot - Anti-CD4 Antibody (A91607) - Antibodies.com Immunofluorescence - Anti-CD4 Antibody (A91607) - Antibodies.com Immunofluorescence - Anti-CD4 Antibody (A91607) - Antibodies.com Immunofluorescence - Anti-CD4 Antibody (A91607) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to CD4 for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-CD4 Antibody
Description
Rabbit polyclonal antibody to CD4.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 26-200 of CD4 (NP_000607.1).
Sequence
KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
51 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CD 4, CD4 (L3T4), CD4 antigen, CD4 antigen (p55), CD4 molecule, CD4 receptor, CD4+ Lymphocyte deficiency, included, CD4mut, CD4_HUMAN, L3T4, Leu3, Ly-4, Lymphocyte antigen CD4, MGC165891, OTTHUMP00000238897, p55, T cell antigen T4, T cell antigen T4/LEU3, T cell differentiation antigen L3T4, T cell OKT4 deficiency, included, T cell surface antigen T4/Leu 3, T cell surface antigen T4/Leu3, T cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, W3/25, W3/25 antigen
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD4 Antibody (A91607) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD4 Antibody (A91607)
Western blot analysis of extracts of Rat lung, using Anti-CD4 Antibody (A91607) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-CD4 Antibody (A91607) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-CD4 Antibody (A91607)
Western blot analysis of extracts of Mouse spleen, using Anti-CD4 Antibody (A91607) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-CD4 Antibody (A91607) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-CD4 Antibody (A91607)
Western blot analysis of extracts of THP-1 cells, using Anti-CD4 Antibody (A91607) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-CD4 Antibody (A91607) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-CD4 Antibody (A91607)
Immunofluorescence analysis of rat spleen using Anti-CD4 Antibody (A91607) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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