Mouse monoclonal [MEM-241] antibody to CD4 (Alexa Fluor® 700).
Specificity
This antibody recognizes an extracellular epitope of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes ("helper" T-cells) and also on monocytes, tissue macrophages, and granulocytes.
Applications
Flow Cytometry, WB
Dilutions
Flow Cytometry: This reagent is designed for analysis of human blood cells using 4µl reagent / 100µl of whole blood or 106 cells in a suspension.
Reactivity
Human
Immunogen
Two N terminal domains of human CD4 fused to human IgG1 (Fc).
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-241
Isotype
IgG1
Conjugate
Alexa Fluor® 700
Excitation: 702nm, Emission: 723nm
Purification
This antibody is conjugated with Alexa Fluor® 700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 15mM Sodium Azide.
Separation of human CD4 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis of human peripheral whole blood stained with Anti-CD4 Antibody [MEM-241] (Alexa Fluor® 700) (4µl reagent / 100µl of peripheral whole blood).
Proteins predicted to interact with CD4
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.