Mouse monoclonal [MEM-59] antibody to CD43 (Alexa Fluor® 700).
Specificity
This antibody recognizes a neuraminidase-sensitive extracellular epitope on CD43, a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes. It is negative on resting B lymphocytes and erythrocytes.
Applications
Flow Cytometry, WB
Dilutions
Flow Cytometry: This reagent is designed for analysis of human blood cells using 4µl reagent / 100µl of whole blood or 106 cells in a suspension.
Reactivity
Human
Immunogen
Human T lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-59
Isotype
IgG1
Conjugate
Alexa Fluor® 700
Excitation: 702nm, Emission: 723nm
Purification
This antibody is conjugated with Alexa Fluor® 700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline with 15mM Sodium Azide.