Anti-CD44v5 Antibody [VFF-8] (A281913)

Anti-CD44v5 Antibody [VFF-8]

Mouse monoclonal [VFF-8] antibody to CD44v5.

This antibody recognises an epitope encoded by exon v5 on the variant region of human CD44. CD44 is a type I transmembrane glycoprotein of variable molecular weight ranging from ~90 kDa to ~220 kDa depending on alternate splicing of the variable region exons and on the degree of glycosylation. CD44 is expressed on multiple cell types and is involved in multiple functions including cell-cell interactions and cell-extracellular matrix binding. Hyaluronan, a high molecular weight polysaccharide component of the extracellular matrix acts as the principal ligand for the CD44 receptor. CD44 isoforms containing one or more sequences encoded by the variant region exons have a much more restricted expression pattern both in terms of organ specificity and immune activation. CD44 isoforms bearing the v5 exon product have been implicated in various neoplasms including breast cancer , renal cell carcinoma and cervical cancer metastases. Mouse anti Human CD44v5 antibody has proved a useful reagent for the imunohistochemical or flow cytometric evaluation of CD44 variants containing the v5 exon product expression in normal and neoplastic cells

WB, IHC-P, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:10, IHC-Fr: 1:10, IHC-P: 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Human

Mouse

Monoclonal

VFF-8

IgG1

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD44v5 antibody, clone VFF-8 specifically recognises an epitope encoded by exon v5 on the variant region of human CD44. CD44 is a type I transmembrane glycoprotein of variable molecular weight ranging from ~90 kDa to ~220 kDa depending on alternate splicing of the variable region exons and on the degree of glycosylation. CD44 is expressed on multiple cell types and is involved in multiple functions including cell-cell interactions and cell-extracellular matrix binding. Hyaluronan, a high molecular weight polysaccharide component of the extracellular matrix acts as the principal ligand for the CD44 receptor (Laurent and Fraser 1992).CD44 isoforms containing one or more sequences encoded by the variant region exons have a much more restricted expression pattern both in terms of organ specificity and immune activation (Mackay et al. 1994). CD44 isoforms bearing the v5 exon product have been implicated in various neoplasms including breast cancer (Tempfer et al. 1996), renal cell carcinoma (Wu et al. 2003) and cervical cancer metastases (Kainz et al. 1996).Mouse anti Human CD44v5 antibody has proved a useful reagent for the imunohistochemical or flow cytometric evaluation of CD44 variants containing the v5 exon product expression in normal and neoplastic cells (Ringel et al. 2001)