Anti-CD45 Antibody [2D1] (Alexa Fluor® 647) (A121749)

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This product is discontinued

Anti-CD45 Antibody [2D1] (Alexa Fluor® 647) (A121749) has been discontinued and is no longer available.

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Specifications

Name

Anti-CD45 Antibody [2D1] (Alexa Fluor® 647)

Description

Mouse monoclonal [2D1] antibody to CD45 (Alexa Fluor® 647).

Specificity

This antibody reacts with an extracellular epitope of all alternative forms of human CD45 antigen, a 180-220 kDa single chain type I transmembrane protein expressed at high levels on all cells of hematopoietic origin, except from erythrocytes and platelets.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: This reagent is designed for analysis of human blood cells using 4µl reagent / 100µl of whole blood or 10⁶ cells in a suspension.

Reactivity

Human

Immunogen

Human peripheral blood mononuclear cells.

Host

Mouse

Clonality

Monoclonal

Clone ID

2D1

Isotype

IgG1

Conjugate

Alexa Fluor® 647

Excitation: 650nm, Emission: 665nm

Purification

This antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 15mM Sodium Azide.

Storage

Store in the dark at 2-8°C. Do not freeze!

Synonyms

L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200

Isotype Controls

Mouse IgG1 [MOPC-21] (Alexa Fluor® 647) (A18746)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Flow Cytometry - Anti-CD45 Antibody [2D1] (Alexa Fluor® 647) (A121749)

Flow cytometric analysis of human peripheral whole blood stained with Anti-CD45 Antibody [2D1] (Alexa Fluor® 647) (4µl reagent / 100µl of peripheral whole blood).

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Flow Cytometry - Anti-CD45 Antibody [2D1] (Alexa Fluor® 647) (A121749)

Separation of human CD45 positive lymphocytes (red-filled) from human CD45 negative blood debris (black-dashed) in flow cytometry analysis of human peripheral whole blood stained with Anti-CD45 Antibody [2D1] (Alexa Fluor® 647) (4µl reagent / 100µl of peripheral whole blood).