Mouse monoclonal [2D1] antibody to CD45 (Pacific Orange).
Specificity
This antibody reacts with an extracellular epitope of all alternative forms of human CD45 antigen, a 180-220 kDa single chain type I transmembrane protein expressed at high levels on all cells of hematopoietic origin, except from erythrocytes and platelets.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: This reagent is designed for analysis of human blood cells using 4µl reagent / 100µl of whole blood or 106 cells in a suspension.
Reactivity
Human
Immunogen
Human peripheral blood mononuclear cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
2D1
Isotype
IgG1
Conjugate
Pacific Orange
Excitation: 400nm, Emission: 551nm
Purification
This antibody is conjugated with Pacific Orange under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 15mM Sodium Azide.
Storage
Store in the dark at 2-8°C. Do not freeze!
Synonyms
L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Separation of human CD45 positive lymphocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis of human peripheral whole blood stained with Anti-CD45 Antibody [2D1] (Pacific Orange) (4µl reagent / 100µl of peripheral whole blood).
Proteins predicted to interact with CD45
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.