This antibody recognises a high molecular weight band of the leucocyte common antigen. MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labeling in bone marrow and none on thymocytes.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat - 1:10, This antibody does not always give a clear separation between positives and negatives in some cell preparations. If users find this to be the case we recommend the use of R. Phycoerythrin conjugated reagent MCA340PE which generally exhibits a peak of higher mean fluorescence. Use 10µl of the suggested working dilutions to label 10⁶ cells in 100µl.

Reactivity

Rat

Immunogen

Purified rat spleen L-CA.

Host

Mouse

Clonality

Monoclonal

Clone ID

OX-33

Isotype

IgG1

Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification

Protein G affinity chromatography of tissue culture supernatant.

Concentration

100 µg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

General Notes

Mouse anti Rat CD45RA antibody, clone OX-33 is directed against a high molecular weight band of the leucocyte common antigen. MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labeling in bone marrow and none on thymocytes (Barclay et al. 1987).

Synonyms

B220, CD45, CD45 antigen, CD45R, EC 3.1.3.48, GP180, L-CA, LCA, Leukocyte common antigen, Loc, Ly-5, LY5, Ly5, homolog of, Lymphocyte antigen 5, Lyt-4, Protein tyrosine phosphatase, receptor type C, Protein tyrosine phosphatase, receptor type, C, Protein tyrosine phosphatase, receptor type, c polypeptide, Ptprc, PTPRC_HUMAN, Receptor-type tyrosine-protein phosphatase C, RT7, T200, T200 glycoprotein, T200 leukocyte common antigen

Isotype Controls