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Anti-CD47 Antibody [ARC51804] (A306258)

Western Blot - Anti-CD47 Antibody [ARC51804] (A306258) - Antibodies.com Western Blot - Anti-CD47 Antibody [ARC51804] (A306258) - Antibodies.com
This product is discontinued

Anti-CD47 Antibody [ARC51804] (A306258) has been discontinued and is no longer available.

View all Anti-CD47 Antibodies.

Name
Anti-CD47 Antibody [ARC51804]
Description
Rabbit monoclonal [ARC51804] antibody to CD47.
Applications
WB, Flow Cytometry
Dilutions
WB: 1:500-1:1,000, Flow Cytometry: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 224-323 of human CD47 (NP_001768.1).
Sequence
HYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALAQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC51804
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
45 - 50 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Antigen identified by monoclonal, Antigenic surface determinant protein OA3, CD 47, CD47 antigen, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, CD47 molecule, CD47_HUMAN, IAP, Integrin Associated Protein, Integrin associated signal transducer, Integrin-associated protein, Leukocyte surface antigen CD47, MER 6, MER6, OA 3, OA3, OTTHUMP00000041152, OTTHUMP00000041153, Protein MER6, Rh related antigen, Surface antigen identified by monoclonal
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD47 Antibody [ARC51804] (A306258) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD47 Antibody [ARC51804] (A306258)
Western blot analysis of extracts of various cell lines, using Anti-CD47 Antibody [ARC51804] (A306258) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-CD47 Antibody [ARC51804] (A306258) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-CD47 Antibody [ARC51804] (A306258)
Western blot analysis of extracts of Jurkat cells, using Anti-CD47 Antibody [ARC51804] (A306258) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
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