This antibody reacts with CD52, a 21-28 kDa extracellular glycoprotein containing a large N-linked carbohydrate moiety. Mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes / macrophages, and in male reproductive tract.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Human tonsil.

Host

Mouse

Clonality

Monoclonal

Clone ID

HI186

Isotype

IgG2b

Conjugate

APC

Excitation: 645nm, Emission: 660nm

Purification

This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Predicted MW

21-28 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Synonyms

Cambridge pathology 1 antigen, CAMPATH 1, CAMPATH 1 antigen, CAMPATH-1 antigen, CD 52, CD52 antigen, CD52 molecule, CD52_HUMAN, CDw52, CDW52 antigen, Epididymal secretory protein E5, Epididymis secretory sperm binding protein Li 171mP, HE 5, He5, Human epididymis-specific protein 5

Isotype Controls