Mouse monoclonal [MEM-53] antibody to CD53 (FITC).
Specificity
This antibody recognises the human CD53 cell surface antigen, also known as Tetraspanin-25. CD53 is a 219 amino acid multi pass transmembrane glycoprotein of 32-42 kDa. CD53 is expressed by all leucocytes, but is absent from erythrocytes and platelets.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1 x 106 cells in 100µl.
Reactivity
Human
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-53
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Ion exchange chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human CD53 antibody, clone MEM-53 recognizes the human CD53 cell surface antigen, also known as Tetraspanin-25. CD53 is a 219 amino acid multi pass transmembrane glycoprotein of 32-42 kDa. CD53 is expressed by all leucocytes, but is absent from erythrocytes and platelets.