Mouse monoclonal [MEM-53] antibody to CD53 (Pacific Blue).
Specificity
This antibody reacts with an extracellular epitope of CD53, a 32-40 kDa tetraspanin family glycoprotein exclusively expressed on leukocytes. It is not present on platelets, red blood cells, and non-hematopoietic cells. This antibody also reacts with the deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Applications
Flow Cytometry
Dilutions
Flow Cytometry: This reagent is designed for analysis of human blood cells using 4µl reagent / 100µl of whole blood or 106 cells in a suspension.
Reactivity
Human
Immunogen
Leukocytes of a patient suffering from LGL Type Leukaemia.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-53
Isotype
IgG1
Conjugate
Pacific Blue
Excitation: 410nm, Emission: 455nm
Purification
This antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 15mM Sodium Azide.