Anti-CD58 Antibody [MEM-63] (FITC) (A281168)

Anti-CD58 Antibody [MEM-63] (FITC)

Mouse monoclonal [MEM-63] antibody to CD58 (FITC).

This antibody recognises human CD58, also known as LFA-3. CD58 is a membrane glycoprotein of ~55-70 kDa. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. CD58 is a heavily N-glycosylated cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.

Flow Cytometry: Neat, Use 20µl of the suggested working dilution to label 10⁶ cells in 100µl

Human

Mouse

Monoclonal

MEM-63

IgG1

Protein A affinity chromatography of tissue culture supernatant.

Lot Specific

Liquid

Supplied in TRIS Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Human CD58 antibody, clone MEM-63 recognizes human CD58, also known as LFA-3. CD58 is a membrane glycoprotein of ~55-70 kDa. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. CD58 is a heavily N-glycosylated cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes (Makgoba et al. 1989). CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.

Ag3, LFA3, Lymphocyte function-associated antigen 3, Surface glycoprotein LFA-3

This product is for research use only. It is not intended for diagnostic or therapeutic use.