Anti-CD6 / T12 Antibody [MIL8] (A281211)

Anti-CD6 / T12 Antibody [MIL8]

Mouse monoclonal [MIL8] antibody to CD6 / T12.

This antibody recognises pig wCD6, the porcine homolog of human CD6.Clone MIL8 was clustered as wCD6 at the Second International Swine CD Antigen Workshop based on its staining pattern of various cell types which was in complete concordance with those exhibited by the reference antibody for the wCD6 cluster at the second workshop, a38b2 (MCA6047). Clone MIL8 and clone a38b2 immunoprecipitated a monomeric protein of ~150 kDa from an activated T-cell lysate. Further, it was established that clones MIL8 and a38b2 bind to different epitopes on porcine wCD6 due to their total lack of reciprocal binding to wCD6 expressing cells.

Flow Cytometry, IHC-Fr

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Porcine

Porcine Lamina Propria Leucocytes.

Mouse

Monoclonal

MIL8

IgG2a

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Pig wCD6, clone MIL8 reacts with pig wCD6, the porcine homolog of human CD6.Clone MIL8 was clustered as wCD6 at the Second International Swine CD Antigen Workshop based on its staining pattern of various cell types which was in complete concordance with those exhibited by the reference antibody for the wCD6 cluster at the second workshop, a38b2 (MCA6047). Clone MIL8 and clone a38b2 immunoprecipitated a monomeric protein of ~150 kDa from an activated T-cell lysate. Further, it was established that clones MIL8 and a38b2 bind to different epitopes on porcine wCD6 due to their total lack of reciprocal binding to wCD6 expressing cells (Pescovitz et al. 1998).