Anti-CD63 Antibody [ARC51703] (A305333)

Name: Anti-CD63 Antibody [ARC51703]
Brand: Antibodies.com
SKU: A305333
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC51703] antibody to CD63 for WB and IHC.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody Lysosome Marker

Specifications

Name

Anti-CD63 Antibody [ARC51703]

Description

Rabbit monoclonal [ARC51703] antibody to CD63.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 103-203 of human CD63 (NP_001771.1).

Sequence

AGYVFRDKVMSEFNNNFRQQMENYPKNNHTASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51703

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

30 - 65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CD 63, CD63 antigen, CD63 antigen (melanoma 1 antigen), CD63 antigen melanoma 1 antigen, CD63 molecule, CD63_HUMAN, gp55, Granulophysin, LAMP 3, LAMP-3, LAMP3, LIMP, Lysosomal associated membrane protein 3, Lysosomal-associated membrane protein 3, Lysosome associated membrane glycoprotein 3, Mast cell antigen AD1, ME491, Melanoma 1 antigen, Melanoma associated antigen ME491, Melanoma associated antigen MLA1, Melanoma-associated antigen ME491, MGC72893, MLA 1, MLA1, NGA, Ocular melanoma associated antigen, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Tetraspanin 30, Tetraspanin-30, Tspan 30, Tspan-30, TSPAN30

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-CD63 Antibody [ARC51703] (A305333)

Western blot analysis of A375, using Anti-CD63 Antibody [ARC51703] (A305333) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-CD63 Antibody [ARC51703] (A305333)

Western blot analysis of various lysates, using Anti-CD63 Antibody [ARC51703] (A305333) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 3: Western Blot - Anti-CD63 Antibody [ARC51703] (A305333)

Western blot analysis of extracts from wild type(WT) and CD63 knockout (KO) 293T cells, using Anti-CD63 Antibody [ARC51703] (A305333) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 4: Immunohistochemistry - Anti-CD63 Antibody [ARC51703] (A305333)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-CD63 Antibody [ARC51703] (A305333) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.