Anti-CD63 Antibody [NKI/C3] (CF® 488) (A251166)

This product is discontinued

Anti-CD63 Antibody [NKI/C3] (CF® 488) (A251166) has been discontinued and is no longer available.

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Name
Anti-CD63 Antibody [NKI/C3] (CF® 488)
Description
Mouse monoclonal [NKI/C3] antibody to CD63 (CF® 488).
Specificity
This antibody recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Applications
Flow Cytometry, IF
Dilutions
Flow Cytometry: 5 µl / million cells or 5 µl / 100 µl whole blood, IF: 1:50-1:100
Reactivity
Human, Mouse
Immunogen
Smooth plasma membrane fraction of MeWo cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
NKI/C3
Isotype
IgG1
Light Chains
kappa
Conjugate

CF® 488

Excitation: 490nm, Emission: 515nm

Purification
Protein A/G chromatography.
Concentration
100 µg/ml
Molecular Weight
26 kDa (core protein) / 30-60 kDa (glycosylated)
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Store at 4-8°C. Antibody is stable for 24 months.
Synonyms
CD 63, CD63 antigen, CD63 antigen (melanoma 1 antigen), CD63 antigen melanoma 1 antigen, CD63 molecule, CD63_HUMAN, gp55, Granulophysin, LAMP 3, LAMP-3, LAMP3, LIMP, Lysosomal associated membrane protein 3, Lysosomal-associated membrane protein 3, Lysosome associated membrane glycoprotein 3, Mast cell antigen AD1, ME491, Melanoma 1 antigen, Melanoma associated antigen ME491, Melanoma associated antigen MLA1, Melanoma-associated antigen ME491, MGC72893, MLA 1, MLA1, NGA, Ocular melanoma associated antigen, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Tetraspanin 30, Tetraspanin-30, Tspan 30, Tspan-30, TSPAN30
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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