Figure 1: Western Blot - Anti-CD68 Antibody [ARC51150] (A308975)
Western blot analysis of extracts of various cell lines, using Anti-CD68 Antibody [ARC51150] (A308975) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Figure 2: Western Blot - Anti-CD68 Antibody [ARC51150] (A308975)
Western blot analysis of extracts of various cell lines, using Anti-CD68 Antibody [ARC51150] (A308975) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Flow cytometry analysis of U-87MG cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-CD68 Antibody [ARC51150] (A308975), (10 µg/ml orange line), followed by FITC conjugated goat anti-Rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained U-87MG cells, used as blank control (red line).
Flow cytometry analysis of Jurkat cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-CD68 Antibody [ARC51150] (A308975), (2.5 µg/ml orange line), followed by FITC conjugated goat anti-Rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained Jurkat cells, used as blank control (red line).
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