Anti-CD68 Antibody [ARC51158] (A308974)

Name: Anti-CD68 Antibody [ARC51158]
Brand: Antibodies.com
SKU: A308974

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This product is discontinued

Anti-CD68 Antibody [ARC51158] (A308974) has been discontinued and is no longer available.

View all Anti-CD68 Antibodies.

Highlighted products for Anti-CD68 Antibody

(6)

A250759

Anti-CD68 Antibody [C68/684]

Mouse monoclonal [C68/684] antibody to CD68 for WB, IF, Flow Cytometry and IHC-P.

(6)

A250759

Anti-CD68 Antibody [C68/684]

Mouse monoclonal [C68/684] antibody to CD68 for WB, IF, Flow Cytometry and IHC-P.

Specifications

Name

Anti-CD68 Antibody [ARC51158]

Description

Rabbit monoclonal [ARC51158] antibody to CD68.

Applications

WB, IHC, Flow Cytometry

Dilutions

WB: 1:500-1:1,000, IHC: 1:10,000-1:80,000, Flow Cytometry: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CD68 (NP_001242.2).

Sequence

TSQGPSTATHSPATTSHGNATVHPTSNSTATSPGFTSSAHPEPPPPSPSPSPTSKETIGDYTWTNGSQPCVHLQAQIQIRVMYTTQGGGEAWGISVLNPNK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51158

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

80 - 130 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CD 68, CD68 antigen, CD68 molecule, CD68_HUMAN, DKFZp686M18236, gp11, Gp110, LAMP4, MACROPHAGE ANTIGEN CD68, Macrophage antigen CD68 (microsialin), Macrosialin, SCARD1, Scavenger receptor class D member 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-CD68 Antibody [ARC51158] (A308974)

Western blot analysis of various lysates, using Anti-CD68 Antibody [ARC51158] (A308974) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-CD68 Antibody [ARC51158] (A308974)

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Figure 3: Immunohistochemistry - Anti-CD68 Antibody [ARC51158] (A308974)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-CD68 Antibody [ARC51158] (A308974) at a dilution of 1:70000(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-CD68 Antibody [ARC51158] (A308974)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CD68 Antibody [ARC51158] (A308974) at a dilution of 1:70000(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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