Anti-CD70 Antibody (A11503)

Name: Anti-CD70 Antibody
Brand: Antibodies.com
SKU: A11503
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to CD70 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-CD70 Antibody

Description

Rabbit polyclonal antibody to CD70.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 47-126 of human CD70 (NP_001243.1).

Sequence

QLPLESLGWDVAELQLNHTGPQQDPRLYWQGGPALGRSFLHGPELDKGQLRIHRDGIYMVHIQVTLAICSSTTASRHHPT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CD 27L, CD 70, CD27 L, CD27 LG, CD27 ligand, CD27-L, CD27L, CD27LG, CD70 antigen, CD70 molecule, CD70_HUMAN, Ki 24 antigen, Ki24 antigen, Surface antigen CD70, TNFSF 7, TNFSF7, Tumor necrosis factor (ligand) superfamily, member 7, Tumor necrosis factor ligand superfamily member 7

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-CD70 Antibody (A11503)

Western blot analysis of extracts of various cell lines, using Anti-CD70 Antibody (A11503) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-CD70 Antibody (A11503)

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Figure 3: Immunohistochemistry - Anti-CD70 Antibody (A11503)

Immunohistochemistry analysis of paraffin-embedded human spleen using Anti-CD70 Antibody (A11503) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-CD70 Antibody (A11503)

Immunofluorescence analysis of 293T cells using Anti-CD70 Antibody (A11503) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).