This antibody recognises an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45 kDa protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signaling following antigen-binding to surface immunoglobulin.

Applications

Flow Cytometry

Dilutions

FC 1: Neat, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Reactivity

Human, Mouse, Rabbit, Horse, Pig, Monkey, Rat, Bovine, Guinea Pig, Fallow deer, American Bison, Red deer, Ferret, Goat

Immunogen

Synthetic peptide corresponding to amino acids 202-216 of human mb-1.

Sequence

ISRGLQGTYQDVGSL

Host

Mouse

Clonality

Monoclonal

Clone ID

HM57

Isotype

IgG1

Conjugate

Excitation: 565nm, Emission: 578nm

Purification

Protein A affinity chromatography of tissue culture supernatant.

Concentration

Reconstitution dependent.

Product Form

Lyophilized

Reconstitution

Pack Size of 100 Tests: Reconstitute with 1 ml of distilled water. Pack Size of 25 Tests: Reconstitute with 0.25 ml of distilled water.

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Storage

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

General Notes

Mouse anti Human CD79a antibody, clone HM57 recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45 kDa protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signaling following antigen-binding to surface immunoglobulin.

Synonyms

B lymphocyte-specific MB1 protein, B-cell antigen receptor complex-associated protein alpha chain, CD 79a, CD79A antigen, CD79a antigen (immunoglobulin-associated alpha), CD79a molecule, immunoglobulin-associated alpha, CD79A_HUMAN, Ig alpha, Ig-alpha, IGA, IgM-alpha, Immunoglobulin-associated alpha, Ly54, MB-1 membrane glycoprotein, MB1, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein

Isotype Controls