Mouse monoclonal [LT8] antibody to CD8 (Pacific Blue).
Specificity
This antibody recognizes an extracellular epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. This antibody blocks Leu2 binding.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension.
Reactivity
Non-Human Primates, Human
Immunogen
Human blood lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
LT8
Isotype
IgG1
Conjugate
Pacific Blue
Excitation: 410nm, Emission: 455nm
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Separation of human CD8 positive lymphocytes (red-filled) from CD8 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-CD8 Antibody [LT8] (Pacific Blue) (4 µl reagent / 100 µl of peripheral whole blood).