Anti-CD8 Antibody [YCATE55.9] (PE) (A282026)

Anti-CD8 Antibody [YCATE55.9] (PE)

Rat monoclonal [YCATE55.9] antibody to CD8 (PE).

This antibody recognises a rat cell line transfected with cDNA for canine CD8a and blocks MHC class I dependant T-cell responses in vitro and in vivo.Rat anti Dog CD8, clone YCATE55.9 has been shown to deplete circulating CD8+ T cells when administered to dogs in vivo. Reduced levels of circulating CD8+ T cells has been associated with decreased survival times for dogs with osteosarcoma.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1 x 10⁶ cells in 100µl

Canine

Canine CD8 alpha chimaeric human IgG1 Fc fusion protein.

Rat

Monoclonal

YCATE55.9

IgG1

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Dog CD8 antibody, clone YCATE55.9 was clustered as Canine CD8 in the First Canine Leukocyte Antigen Workshop (Cobbold et al. 1994). YCATE55.9 reacts with a rat cell line transfected with cDNA for canine CD8a (Gorman et al. 1994) and blocks MHC class I dependant T-cell responses in vitro and in vivo.Rat anti Dog CD8, clone YCATE55.9 has been shown to deplete circulating CD8+ T cells when administered to dogs in vivo. (Watson et al. 1993) Reduced levels of circulating CD8+ T cells has been associated with decreased survival times for dogs with osteosarcoma (Biller et al. 2010).

CD8 antigen, alpha polypeptide (p32), CD8a, CD8a antigen, CD8a molecule, CD8A_HUMAN, Leu2, Leu2 T lymphocyte antigen, MAL, OKT8 T cell antigen, p32, T cell antigen Leu2, T cell co receptor, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, T8 T cell antigen

This product is for research use only. It is not intended for diagnostic or therapeutic use.