Anti-CD80 Antibody (A90634)

Name: Anti-CD80 Antibody
Brand: Antibodies.com
SKU: A90634
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to CD80 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-CD80 Antibody

Description

Rabbit polyclonal antibody to CD80.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD80 (NP_005182.1).

Sequence

MGHTRRQGTSPSKCPYLNFFQLLVLAGLSHFCSGVIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 - 75 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Activation B7-1 antigen, B lymphocyte activation antigen B7, B7, B7-1, B7-1 antigen, B7.1, BB1, CD28 antigen ligand 1, CD28LG, CD28LG1, CD80 antigen, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), CD80 molecule, CD80_HUMAN, Costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-CD80 Antibody (A90634)

Western blot analysis of extracts of Rat lung, using Anti-CD80 Antibody (A90634) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Western Blot - Anti-CD80 Antibody (A90634)

Western blot analysis of various lysates, using Anti-CD80 Antibody (A90634) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

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Figure 3: Immunohistochemistry - Anti-CD80 Antibody (A90634)

Immunohistochemistry analysis of paraffin-embedded human spleen using Anti-CD80 Antibody (A90634) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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