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Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327)

Western Blot - Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327) - Antibodies.com Immunohistochemistry - Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327) - Antibodies.com Immunohistochemistry - Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327) - Antibodies.com Immunohistochemistry - Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327) - Antibodies.com
This product is discontinued

Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327) has been discontinued and is no longer available.

View all Anti-CD81 Antibodies.

Name
Anti-CD81 Antibody [ARC57928 + ARC57931]
Description
Rabbit Oligoclonal [ARC57928 + ARC57931] antibody to CD81.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein of human CD81.
Host
Rabbit
Clonality
Oligoclonal
Clone ID
ARC57928 + ARC57931
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
20 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
26 kDa cell surface protein TAPA 1, 26 kDa cell surface protein TAPA-1, 26 kDa cell surface protein TAPA1, CD 81, CD81 antigen, CD81 antigen (target of antiproliferative, CD81 molecule, CD81_HUMAN, CVID6, S5.7, TAPA 1, TAPA1, Target of the antiproliferative, Tetraspanin 28, Tetraspanin-28, Tetraspanin28, Tspan 28, Tspan-28, Tspan28
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327)
Western blot analysis of extracts of various lysates, using Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327)
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327)
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327)
Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-CD81 Antibody [ARC57928 + ARC57931] (A309327) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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